Diagnostic use of the TMA technology
To use the Tissue Microarray Technology for diagnostic purposes means that you are able to control the analytical identification error (by which is intended the error introduced by coring donor block, allocating cores in the matrix and read individual cores as belonging to one patient).
We have worked extensively with Integrated System Engineering, Aperio (represented in Italy until June 2013 by Nikon, then part of Leica Microsystems) and Noemalife to bring to a negligible amount this type of error.
Our manuscript, entitled "Tissue Microarray design and construction for
scientific, industrial and diagnostic use" has been published open access in the Journal of Pathology Informatics.
Following our JPI paper, we developed (August 2013) an open-source platform to visualize patient's cores and create e a database for scoring record keeping.
This platform is an ID error controlled, clinical grade tool for pathologists to sign-out from their desk immunostains performed on their cases.
In addition, allows freeware image analysis of the images in bulk, provided the scanning and segmenting program produces imges of the individual cores.
A short presentation illustrating the requirements, the solutions and the platform can be downloaded here.
The platform is free and chan be shared upon signin a cooperative agreement.
Here below are the requirements for a clinical grade, TMA platform:
Unique ID on all objects (slides, tissue blocks, patients, samples, etc.).
Linear or 2D barcode on: slide labels or tissue blocks, TMA slide labels, TMA blocks.
A program to extract patient's and/or specimen ID from the LIS when prompted by the glass slide or tissue block barcode.
A Tissue MicroArrayer with barcode-driven donor tissue check and random core placement in the design.
A solid, asymmetric TMA design.
A Tissue MicroArrayer able to produce and export the TMA design in an XML format.
A whole slide scanner with enabled barcode reader, ability to import the TMA arrayer XML design files, matching of the TMA design unique ID with the ID on the TMA slide label.
A de-arraying (segmenting) program with a grid-like representation of the TMA design.
Ability to export an image of each core, tagged with the TMA ID, the stain and the row and column position in the filename.
An image database searchable for patient ID's and IHC stains, in multiple fields.
Please do not hesitate to contact us at:
Prof. Giorgio Cattoretti, MD, FCAP, ABP certified.
Associate Professor, Università degli Studi Milano-Bicocca. Director of the Residency Program in Anatomic Pathology.
Director of the Department of Surgical Pathology, Cytology, Medical Genetics and Nephropathology.
Azienda Ospedaliera San Gerardo
Via Pergolesi 33, 20052, Monza (MI)
+39 039 233-2551 phone
+39 039 233-2548 FAX