Instrumentation

The Histopathology Division of the Surgical Pathology Department (Dept. of Surgical Science - University Milano- Bicocca) has a fully operational modern Histopathology service instrumentation (continuous automated processors, embedding stations, microtomes and autostainers). The TMA facility is a top notch, state of the art set of instruments to allow production and high-throughput analysis of Tissue Microarrays, for protein and gene (FISH) expression, image analysis and quantitation.

1. Computer Assisted Tissue Microarrayer Model TMA Galileo CK4500 ( Integrated Systems Engineering srl, Milano)*, inclusive of the TMA Software ISE.
The use of relatively small tissue cores, positioned onto a unique slide, allows simultaneous histological analysis of hundreds of samples.
High throughput screening of expression
Reduction of the number of slides to be mounted and analyzed >Time saving
Reduction of the amount of reagents and antibodies used > Money saving
Analysis of various biological specimens under uniform reaction conditions > Increasing the consistency of the results
Saving of precious biological samples and possibility to re-use donor blocks

HIGH SPEED (x8) TMA creation.
TISSUE CORE PICKING & DISPENSING
Galileo 4500 can be used for TMA, CMA and as sample picker for nucleic acids (DNA and RNA) extraction and for protein isolation using appropriate fixative.
The tissue cores extracted from precise regions of interest may be dispensed into dedicated vessels for further processing such as DNA, RNA, miRNA and protein extraction. Microfuge vials, strips or microtiter plates may be used, depending on the downstream applications. Parallel analyses on the same specimen can be performed simultaneously.

Interfacie with ScanScope Aperion and with the Histopathology reporting LIS (Athena, Noemalife).
Fully anonimized or coded pathology samples are available. Coded samples availability is subject to filing a project within the HSG IRB (Comitato Etico Indipendente).

We are the first TMA facility abilitated for diagnostic work with TMA, because of the linear barcode-controlled, error-free match between the core images and the sample ID. (Pilla D, et al., Journal of Pathology Informatics, July 2012, submitted).

2. ScanScope FL System ( Aperio Technologies)* inclusive of:
  • Five slides tray
  • 4 color + immunofluorescence virtual slide scanning.
  • Software Spectrum Plus
  • Digital Pathology Information Management Software
  • TMA II software for automatic Tissue micro array analysis.
  • 3. ScanScope CS System** ( Aperio Technologies) inclusive of:
  • Five slides tray
  • Software Spectrum Plus
  • Digital Pathology Information Management Software
  • TMA II software for automatic Tissue micro array analysis.
  • Personnel

    The lab is located within a busy, professional Division of Pathology, fully staffed, processing >25.000 specimens/year.
    The Division has a Clinical Genetics sections, with a pre-natal and a tumor genetic labs, analyzing 1800 karyotypes/year and >600 FISH preparations.
    The TMA facility has an extensive Mouse Pathology experience. A Mouse Pathology website created by Prof. Cattoretti is hosted by Columbia University, New York, NY, USA.

    * These instruments have been financed by a Regione Lombardia Research Grant (Decree N. 13848 on 12/11/2009, Project: "Diagnostica ad alta efficienza per la terapia oncologica personalizzata." or High Throughput Diagnostics.)
    ** these instruments have been acquired with startup funds from a contract for renal histopathology international review, signed by Dr. Franco Ferrario (Nephropathology, HSG) and Human Genomic Sciences, USA.