TMA tools


Here you will find useful tools and rerefences on how to optimize the use of Tissue Microarray.

WHAT YOU NEED TO CONSIDER WHEN ORDERING OR CREATING A TMA
HOW TO SELECT CORE NUMBER AND DIAMETER FOR TMAs
Here below is an algorithm (downloadable) which will guide you in choosing the right size and core numbers, maximizing your tissue availability while avoiding damaging the diagnostic power of the original tissue block.


This algorithm was derived from the following literature references:
1. Rimm, D. L. et al. Cancer and leukemia group B pathology committee guidelines for tissue microarray construction representing multicenter prospective clinical trial tissues. J Clin Oncol 29, 22822290 (2011).
2. Goethals, L. et al. A new approach to the validation of tissue microarrays. J Pathol 208, 607614 (2006).
3. Camp, R. L., Charette, L. A. & Rimm, D. L. Validation of tissue microarray technology in breast carcinoma. Lab Invest 80, 19431949 (2000).
4. Nocito, A. et al. Microarrays of bladder cancer tissue are highly representative of proliferation index and histological grade. J Pathol 194, 349357 (2001).
5. Anagnostou, V. K., Lowery, F. J., Syrigos, K. N., Cagle, P. T. & Rimm, D. L. Quantitative evaluation of protein expression as a function of tissue microarray core diameter: is a large (1.5 mm) core better than a small (0.6 mm) core? Arch Pathol Lab Med 134, 613619 (2010).
6. Collins, L. C. et al. Potential role of tissue microarrays for the study of biomarker expression in benign breast disease and normal breast tissue. Appl. Immunohistochem. Mol. Morphol. 17, 438441 (2009).
7. Camp, R. L., Neumeister, V. & Rimm, D. L. A decade of tissue microarrays: progress in the discovery and validation of cancer biomarkers. J Clin Oncol 26, 56305637 (2008).